Read Single-Molecule Sequencing of RNA

Single-Molecule Sequencing of RNA

Dina Grohmann

4.9/5 (18584 ratings)

Description:What biological questions can single-molecule RNA sequencing help address that traditional methods cannot? How can these technologies be applied in your area of research, and what limitations might you encounter? Are specific bioinformatics tools or experimental protocols particularly suited to your needs? Single-Molecule Sequencing of RNA provides the reader with answers to these questions. Recent advances in RNA sequencing technologies have revolutionized the field of transcriptomics, shedding new light on the complexities of RNA biology. Single-molecule RNA sequencing, in particular, has opened unprecedented avenues for exploring gene expression, isoform diversity, RNA modifications, and dynamic transcript behaviors at single-molecule resolution. This transition from bulk RNA analyses to single-molecule studies represents a paradigm shift, enabling researchers to study the transcriptome with unparalleled accuracy and detail one molecule at a time. With this primer, the authors provide an understandable and comprehensive introduction to single-molecule RNA sequencing, beginning with a historical perspective and progressing to the latest technological developments. Key topics include principles of the currently most relevant third-generation single-molecule platforms from PacBio and Oxford Nanopore Technologies, developments on RNA library preparation, sequencing workflows, data analysis pipelines, and examples of real-world applications. Throughout the chapters, the authors emphasize both the opportunities and challenges presented by single-molecule RNA-seq, from its technical limitations to its unique capabilities in resolving full-length transcripts, detecting rare isoforms, and mapping epitranscriptomic modifications.We have made it easy for you to find a PDF Ebooks without any digging. And by having access to our ebooks online or by storing it on your computer, you have convenient answers with Single-Molecule Sequencing of RNA. To get started finding Single-Molecule Sequencing of RNA, you are right to find our website which has a comprehensive collection of manuals listed.
Our library is the biggest of these that have literally hundreds of thousands of different products represented.

Pages: 164

Format: PDF, EPUB & Kindle Edition

Publisher: American Chemical Society

Release: 2025

ISBN: MwtZEQAAQBAJ

Description: What biological questions can single-molecule RNA sequencing help address that traditional methods cannot? How can these technologies be applied in your area of research, and what limitations might you encounter? Are specific bioinformatics tools or experimental protocols particularly suited to your needs? Single-Molecule Sequencing of RNA provides the reader with answers to these questions. Recent advances in RNA sequencing technologies have revolutionized the field of transcriptomics, shedding new light on the complexities of RNA biology. Single-molecule RNA sequencing, in particular, has opened unprecedented avenues for exploring gene expression, isoform diversity, RNA modifications, and dynamic transcript behaviors at single-molecule resolution. This transition from bulk RNA analyses to single-molecule studies represents a paradigm shift, enabling researchers to study the transcriptome with unparalleled accuracy and detail one molecule at a time. With this primer, the authors provide an understandable and comprehensive introduction to single-molecule RNA sequencing, beginning with a historical perspective and progressing to the latest technological developments. Key topics include principles of the currently most relevant third-generation single-molecule platforms from PacBio and Oxford Nanopore Technologies, developments on RNA library preparation, sequencing workflows, data analysis pipelines, and examples of real-world applications. Throughout the chapters, the authors emphasize both the opportunities and challenges presented by single-molecule RNA-seq, from its technical limitations to its unique capabilities in resolving full-length transcripts, detecting rare isoforms, and mapping epitranscriptomic modifications.We have made it easy for you to find a PDF Ebooks without any digging. And by having access to our ebooks online or by storing it on your computer, you have convenient answers with Single-Molecule Sequencing of RNA. To get started finding Single-Molecule Sequencing of RNA, you are right to find our website which has a comprehensive collection of manuals listed.
Our library is the biggest of these that have literally hundreds of thousands of different products represented.

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Single-Molecule Sequencing of RNA

Single-Molecule Sequencing of RNA

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